Leucine Dehydrogenase fromCorynebacterium pseudodiphtheriticum:Purification and Characterization
نویسندگان
چکیده
منابع مشابه
isolation and characterization of leucine dehydrogenase from a thermophilic citrobacter freundii jk-91strain isolated from jask port.
background and objectives: leucine dehydrogenase (leudh; ec 1.4.1.9) belongs to the amino acid dehydrogenase family and isused as a biocatalyst in medical and pharmaceutical industries (1). this study reported deals with the isolation and characterization of leudh from a thermophilic bacterium isolated from jask port in the province of hormozgan. materials and methods: aliquots of soil and wate...
متن کاملProperties of crystalline leucine dehydrogenase from Bacillus sphaericus.
The distribution of bacterial leucine dehydrogenase (L-leucine:NAD+ oxidoreductase, deaminating, EC 1.4.1.9) was investigated, and Bacillus sphaericus (IFO 3525) was found to have the highest activity of the enzyme. Leucine dehydrogenase, which was purified to homogeneity and crystallized from B. sphaericus, has a molecular weight of about 245,000 and consists of six identical subunits (Mr = 41...
متن کاملIsolation and characterization of Leucine dehydrogenase from a thermophilic Citrobacter freundii JK-91strain Isolated from Jask Port
BACKGROUND AND OBJECTIVES Leucine dehydrogenase (LeuDH; EC 1.4.1.9) belongs to the amino acid dehydrogenase family and isused as a biocatalyst in medical and pharmaceutical industries (1). This study reported deals with the isolation and characterization of LeuDH from a thermophilic bacterium isolated from Jask Port in the Province of Hormozgan. MATERIALS AND METHODS Aliquots of soil and wate...
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15 صفحه اولPurification and Characterization of Formate Dehydrogenase
Anaylobacter aguaticus strain KNK607M, which had high NAD-dependent formate dehydrogenase (FDH) activity, was newly isolated. The enzyme, purified to homogeneity, was a dimer composed of identical subunits with a molecular mass of 44 kDa. The specific activity was 9.5 ulmg, and the enzyme was optimum at pH 6.3 and 500C, most stabte at pH 7.0, and stable at 50"C or lovver. The apparent K. yalues...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369
DOI: 10.1080/00021369.1990.10870153